Centering test charts is critical for image quality testing. If the camera is not properly aligned with the test chart, your image may not be suitable for analysis; you may not know if the results are truly indicative of the camera system, or from an error in positioning the chart.
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If the optical axis is offset relative to the center of the target, the resolution regions of interest can become asymmetrical, shifting from the ideal radial field distance and introducing error in your map of sharpness across your imaging plane. If the device is tilted relative to the target, measurements of keystone distortion and convergence angles will have reduced accuracy. Controlling the alignment of the camera relative to the test chart is the best practice to prevent these errors.
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